家蚕BmNPV多角体蛋白的表达规律及importinα介导的Polh入核转运

Expression pattern of polyhedrin of BmNPV and involvement of importin α in the nuclear import of Polh

摘要:

家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus,BmNPV)对蚕丝业的危害极大。该病毒的多角体蛋白编码基因polyhedrin(polh)能够在病毒感染极晚期超高水平表达，并在细胞核内形成包涵体，多角体蛋白转运到宿主细胞核中的详细机制目前未知。为阐明病毒蛋白的入核机制，本研究通过分子克隆、免疫共沉淀(co-immunoprecipitation,CO-IP)、免疫荧光等方法，详细分析了多角体蛋白(polyhedrin,Polh)的表达规律，并进一步揭示了宿主蛋白importin α通过蛋白互作的方式参与多角体蛋白的入核转运，为进一步深入阐明包括Polh在内的诸多杆状病毒入核蛋白的入核机制提供了参考。

Abstract:

Bombyx mori nucleopolyhedrovirus (BmNPV) is extremely harmful to the silk industry. The polyhedrin, which encodes the polyhedrin (Polh), can be expressed at ultra-high levels and form occlusion bodies in the nucleus, embedding the progeny virus within it. However, the detailed mechanism by which polyhedrin is transported into the host cell nucleus remains unknown. Clarifying the nuclear import mechanisms of viral proteins can help us develop better prevention and treatment measures against baculoviruses. This study employed molecular cloning, co-immunoprecipitation, and immunofluorescence to analyze in detail the expression pattern of the highly expressed polyhedrin in the very late stage of the virus, and further revealed that the host protein importin α participates in the nuclear import of polyhedrin through protein interactions. This study provides a reference for further elucidating the nuclear import mechanisms of the baculovirus proteins including polyhedrin that can enter the nucleus.