Abstract:The paradigm of higher engineering education in China has transformed from emphasizing knowledge foundation and ability training to emphasizing quality shaping. How to cultivate engineering talents with solid professional knowledge, innovative thinking, and practical ability under the background of emerging engineering education has become a core issue and urgent problem to be solved in the education of various engineering majors. This paper takes Genetic Engineering, a main course in the curriculum system of bioengineering, as an example to explain the reform ideas of implementing the problem-based learning (PBL) model in online-offline blended teaching. Practice has proved that this reform idea not only significantly improves the teaching quality of the course and the learning effect of students but also provides a reference model for cultivating engineering talents with innovative thinking, practical ability, and correct values.