固定化脂肪酶合成维生素A棕榈酸酯

Immobilized Lipase Catalyzed Synthesis of Vitamin A Plamitate

摘要:

研究了有机溶剂中脂肪酶催化维生素A棕榈酸酯的合成工艺。采用维生素A醋酸酯和棕榈酸乙酯作为反应底物, 对催化合成维生素A棕榈酸酯反应介质进行了比较, 同时对影响合成维生素A棕榈酸酯反应的因素(温度、初始水含量、底物摩尔比、反应时间和酶量等)进行了探讨, 优化了反应条件: 在10 mL的石油醚中, 体系初始含水量0.2%(体积比V/V), 0.100 g 维生素A醋酸酯和0.433 g 棕榈酸乙酯在酶量为1.1 g的固定化酶催化下, 在30°C、190 r/min下反应12 h, 转化率可以达到83%, 固定化酶可连续使用5次以上。

Abstract:

The synthesis of vitamin A plamitate in organic solvent with vitamin A acetate and ethyl palmitate with immobilized lipase from Candida sp. was studied. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by transesterification from 0.100 g vitamin A acetate and 0.433 g ethyl palmitic, at 30°C, in 10 mL petroleum ether, containing 0.2% of water (V/V), with 1.1 g lipase. In these conditions, the yield of vitamin A palmitate reached 83% in 12 h. The immobilized lipase was reused about 5 batches.