发状念珠藻胞外多糖的纯化与性质分析

Purification and Characterization of Extracellular Polysaccharides from Nostoc flagelliforme

摘要:

采用DEAE阴离子交换层析和Sephadex G100凝胶层析对液体悬浮培养发状念珠藻胞外多糖进行纯化, 得到两个组分NFPS1和NFPS2。对组分NFPS2进行理化性质分析, 并与野生发状念珠藻多糖NFPS0的性质进行对比。结果表明二者具有相似的单糖组成, 均为葡萄糖、木糖、半乳糖、甘露糖; 表观分子量分别为2.79×105、2.26×105; 均不含核酸、蛋白质等物质, 是非硫酸化多糖; 有较高的热稳定性, 其降解温度在245oC左右。但在微观结构上, 两者存在一定差别。

Abstract:

The extracellular polysaccharides (EPS) of N. flagelliforme were purified by DEAE anion exchange chromatography and Sephadex G100 gel filtration chromatography. And two main components named NFPS1 and NFPS2 were obtained respectively. The physico-chemical characteristics of NFPS2 were analyzed and compared with NFPS0, which was obtained from field colony of N. flagelliforme. These results showed that both of NFPS2 and NFPS0 were composed of four monosaccharides: glucose, xylose, galactose and mannose. The apparent molecular weight of NFPS2 and NFPS0 was estimated to be 2.79×105, 2.26×105 respectively. They are non-sulfated polysaccharides, free of protein and nuclear acid. The thermal analysis indicated that there was a decomposition peak at 245oC in thermogravimetric (TG) curves. However, the microstructure analysis showed that they had different porous structures.