Kozak序列对金黄色葡萄球菌黏附因子FnBPA-A DNA疫苗诱导小鼠免疫应答的影响
DOI:
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金 (Nos. 31160191,31260222),新疆维吾尔自治区普通高校重点学科基础兽医学科项目资助。


Effect of Kozak sequence on mice DNA vaccine immunization of Staphylococcus aureus adhesion fibronectin-binding protein FnBPA-A
Author:
Affiliation:

Fund Project:

National Natural Science Foundation of China (Nos. 31160191, 31260222), Ordinary University Basic Veterinary Medicine Key Disciplines Foundation of Xinjiang Uygur Autonomous Region.

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    黏附因子FnbpA (纤连蛋白结合蛋白A,Fibronectin binding protein A) 是金黄色葡萄球菌表面的蛋白质成分,是该菌感染早期最重要的致病因子,可促进其对寄主组织的侵入,也是一个有潜力的免疫靶标。将牛乳源金黄色葡萄球菌中FnBPA基因的A功能区克隆至真核表达载体中,分别构建含Kozak序列和不含Kozak序列的FnBPA-A基因真核表达载体,重组质粒经鉴定测序正确后,免疫C57BL/6小鼠检测其抗体水平和淋巴细胞增殖情况,并对各组小鼠进行攻毒实验。检测的结果表明Kozak修饰的重组DNA在血清抗体效价 (P<0.05) 和免疫保护率方面均优于不含Kozak序列的重组DNA,在刺激淋巴细胞增殖方面Kozak修饰的重组DNA的刺激效果虽然也高于不经修饰的重组DNA,但是差异不显著 (P>0.05)。根据总体的免疫效果来看,Kozak序列对增强FnBPA的重组DNA疫苗诱导的免疫应答起了不容忽视的作用。

    Abstract:

    Fibronectin-binding protein (FnBPA) is a protein that expresses on cell surface of Staphylococcus aureus during early stage of infection. FnBPA was capable of promoting Staphylococcus aureus to invade cells and was viewed as a potential immune target. Based on the FnBPA-A gene two recombinant expression vectors with or without Kozak sequence were constructed. After identified and confirmed by restriction enzyme digestion and sequencing they were used to immunize C57BL/6 mice. Then induced antibody titer, T lymphocyte proliferative response and experiment mice challenge test were measured. Our result indicates that humoral immune responses and challenge experiment induced by recombinant DNA with Kozak sequence were better than those without Kozak sequence (P<0.05). For T lymphocyte proliferative response the induced effect of recombinant DNA with Kozak sequence was higher than that without Kozak sequence, but there was no significant difference (P>0.05). We conclude that Kozak sequence could play an important role in immune response induced by FnBPA-A recombinant DNA.

    参考文献
    相似文献
    引证文献
引用本文

苏艳,王世民,邵俊高,张宝江,韦海娜. Kozak序列对金黄色葡萄球菌黏附因子FnBPA-A DNA疫苗诱导小鼠免疫应答的影响[J]. 生物工程学报, 2013, 29(4): 458-465

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2012-09-23
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2013-04-03
  • 出版日期:
文章二维码
您是第位访问者
生物工程学报 ® 2025 版权所有

通信地址:中国科学院微生物研究所    邮编:100101

电话:010-64807509   E-mail:cjb@im.ac.cn

技术支持:北京勤云科技发展有限公司