黑曲霉催化雄甾-4-烯-3,17-二酮16β-羟基化

16β-hydroxylation of 4-androstene-3,17-dione by Aspergillus niger

摘要:

为进一步确定黑曲霉菌株TCCC41650的生物转化能力，以雄甾-4-烯-3,17-二酮 (Androstenedione) 为底物，利用黑曲霉菌株TCCC41650进行催化，产物经纯化、重结晶后，通过单晶衍射鉴定为16β-羟基雄甾-4-烯-3,17-二酮。转化条件为：培养液pH 6.0，乙醇添加量为2%，投料浓度为1‰时，72 h转化率为85.8%。目前甾体研究领域对于C16β-羟基化的微生物转化未见报道，研究结果为C16β-羟基甾体药物的研发奠定了 基础。

Abstract:

In order to discover the steroid biotransformation ability of ?lamentous fungus Aspergillus niger TCCC41650, we studied the fermentation of 4-androstene-3,17-dione with A. niger TCCC41650. The transformation product was purified, crystallized and determined as 16β-hydroxy-androst-4-ene-3,17-dione by X-ray single crystal diffraction method. The best fermentation condition was found to be pH 6.0, ethanol amount 2% with a substrate concentration of 1‰, the transformation rate is 85.81% after 72 h. Based on the best of our knowledge, 16β-hydroxylation rarely occurs in microbial transformations of steroid. This study laid the foundation for the research of 16β-hydroxylation steroids.