发酵产丁二酸过程中废弃细胞的循环利用
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国家重点基础研究发展计划 (973计划) (No. 2009CB724701),国家自然科学基金 (No. 20606017),材料化学工程国家重点实验室基金,江苏省“青蓝工程”资助。


Recycle of spent cells from anaerobic succinate fermentation
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National Basic Research Program of China (973 Program) (No. 2009CB724701), National Natural Science Foundation of China (No. 20606017), State Key Laboratory of Materials-Oriented Chemical Engineering, “Qinglan Project” of Jiangsu Province.

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    摘要:

    对厌氧发酵产丁二酸后的废弃细胞进行破壁处理,考察了以细胞水解液作为有机氮源重新用于丁二酸发酵的可行性。比较了超声破碎、盐溶、酶解3种方法破碎细胞获得的水解液作为氮源发酵产丁二酸的效果,结果表明酶解制得的细胞水解液效果最佳。以总氮含量为1.11 g/L的酶解液 (相当于10 g/L酵母膏) 作为氮源发酵,丁二酸产量可达42.0 g/L,继续增大酶解液用量对耗糖、产酸能力没有显著提高。将细胞酶解液与5 g/L酵母膏联用发酵36 h后,丁二酸产量达75.5 g/L,且丁二酸生产强度为2.10 g/(L·h),比

    Abstract:

    Spent cells recovered from anaerobic fermentation by Actinobacillus succinogenes were used as nitrogen source for succinic acid production. Three methods were investigated for cell wall-breaking. The results showed that enzymatic hydrolysis was more effective for higher succinic acid yield. When the enzymatic hydrolysate of spent cells was added to reach a total nitrogen concentration 1.11 g/L (equivalent to 10 g/L yeast extract), the succinic acid concentration was 42.0 g/L, but it increased slightly when enhancing the level of enzymatic hydrolysate. However, when 5 g/L yeast extract was supplemented with the enzymatic hydrolysate of spent cells, the succinic acid concentration reached 75.5 g/L after 36 hours and, the succinic acid productivity was 2.10 g/(L·h), which increased by 66.7% compared with the fermentation using 10 g/L yeast extract. Therefore, enzymatic hydrolysate of spent cells could replace 50% yeast extract in the original medium for succinic acid production.

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白雪飞,陈可泉,叶贵子,黄秀梅,李建,姜岷. 发酵产丁二酸过程中废弃细胞的循环利用[J]. 生物工程学报, 2010, 26(9): 1276-1280

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  • 收稿日期:2010-03-01
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