一株氯嘧磺隆降解菌分离鉴定及降解条件优化
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家重点研发计划 (No. 2018YFD0200800),黑龙江省自然科学基金 (No. C2017055) 资助。


Isolation and identification of a chlorimuron-ethyl-degrading bacterium and optimization of its degradation conditions
Author:
Affiliation:

Fund Project:

National Key R&D Program of China (No. 2018YFD0200800), Natural Science Foundation of Heilongjiang Province of China (No. C2017055).

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为解决氯嘧磺隆残留对土壤、水体污染及后茬敏感作物药害问题,为污染土壤微生物修复提供降解菌种资源,文中采用富集培养、逐级驯化等方法,从氯嘧磺隆污染土壤中分离到1株高效氯嘧磺隆降解菌T9DB-01,经形态特征、生理生化及16S rDNA序列分析,鉴定为假单胞菌Pseudomonas sp.。采用单因素实验探究温度、pH值、底物浓度、装液量和接种量对菌株T9DB-01降解氯嘧磺隆的影响,采用正交试验及验证,优化菌株T9DB-01对氯嘧磺隆降解条件。结果表明,在30 ℃,pH 8.0,底物浓度200 mg/L,装液量100 mL/250 mL,接种量4%的条件下,5 d后降解率达到93.7%。该降解菌株对氯嘧磺隆污染土壤原位生物修复具有一定的应用潜力。

    Abstract:

    In order to solve the problem of soil, water pollution and sensitive crop drug damage caused by chlorosulfuron residue, and to provide degradation strain resources for microbial remediation of contaminated soil, a chlorimuron-ethyl-degrading strain T9DB-01 was isolated from chlorosulfuron contaminated soil by the method of enrichment culture and step by step domestication. Strain T9DB-01 was identified as Pseudomonas sp. by morphological characteristics, physiological and biochemical analysis and 16S rDNA gene sequence analysis. The effects of temperature, pH value, substrate concentration, medium volume, and inoculation volume on the degradation of chlorsulfuron-methyl by strain T9DB-01 were investigated by single factor experiment. The degradation conditions of chlorosulfuron by strain T9DB-01 were optimized by orthogonal test and verification. Results show that 30 °C, pH 8.0, inoculum 4%, liquid volume 100 mL/250 mL, substrate concentration of 200 mg/L, cultured for 5 d, the strain degraded 93.7% chlorsulfuron-methyl. The degrading strain has certain application potential for bioremediation of chlorsulfuron-contaminated soil.

    参考文献
    相似文献
    引证文献
引用本文

杨峰山,张瑞,肖延臣,刘春光,付海燕. 一株氯嘧磺隆降解菌分离鉴定及降解条件优化[J]. 生物工程学报, 2020, 36(3): 560-568

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2019-08-19
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-03-31
  • 出版日期:
您是第位访问者
生物工程学报 ® 2024 版权所有

通信地址:中国科学院微生物研究所    邮编:100101

电话:010-64807509   E-mail:cjb@im.ac.cn

技术支持:北京勤云科技发展有限公司