糠醛对粘红酵母生长与油脂积累的影响
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国家高技术研究发展计划 (863计划) (Nos. 2013AA050702, 2012AA022304) 资助。


Effects of furfural on the growth and lipid production of oleaginous yeast Rhodotorula glutinis
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National High Technology Research and Development Program of China (863 Program) (Nos. 2013AA050702, 2012AA022304).

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    摘要:

    为了探究纤维素水解液中常见的发酵抑制物糠醛对粘红酵母Rhodotorula glutinis生长与油脂积累的影响,对比了不同的糠醛浓度 (0.1 g/L, 0.4 g/L, 0.6 g/L, 1.5 g/L) 下粘红酵母的生物量和油脂积累情况,并探究了1.0 g/L的糠醛对粘红酵母不同碳源 (葡萄糖和木糖) 利用的影响。研究表明,当糠醛浓度达1.5 g/L时,粘红酵母的延迟期延长至96 h,残糖高达17.7 g/L,生物量最高6.6 g/L,仅为正常积累量的47%,油脂含量也减少了约50%;以木糖为碳源时,糠醛对粘红酵母的抑制程度小于葡萄糖为碳源时的情况;在糠醛存在的逆境中,粘红酵母倾向于生成更多的18碳脂肪酸或18碳不饱和脂肪酸。

    Abstract:

    In order to illustrate the effects of furfural, one of the most common inhibitory compounds in lignocellulosic hydrolysate, on oleaginous yeast Rhodotorula glutinis, we investigated the effects of different concentrations of furfural (0.1, 0.4, 0.6 and 1.5 g/L) on the biomass and lipid production of R. glutinis, as well as the effects of 1.0 g/L furfural on the utilization of glucose and xylose. Results showed that: when the furfural concentration reached 1.5 g/L, the lag phrase time was extended to 96 h, and the residual glucose was up to 17.7 g/L, with maximum biomass of only 6.6 g/L, which accounted for 47% of that in the basic medium (furfural-free), and the corresponding lipid content was reduced about 50%. Furfural showed lighter inhibitory degree on R. glutinis when xylose acted as the carbon source than glucose was the carbon source; more C18 fatty acids or unsaturated C18 fatty acids were generated in the presence of furfural.

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雍子涵,张栩,谭天伟. 糠醛对粘红酵母生长与油脂积累的影响[J]. 生物工程学报, 2015, 31(10): 1484-1491

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  • 收稿日期:2014-11-17
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  • 在线发布日期: 2015-10-13
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