Screening for Peptides of Anti-rotavirus by Phage-displayed Technique

Abstract:

In this study，a 15-mer phage display peptide library was employed to pan against human rotavirus immobilized on solid phase. 4 different peptides were selected and could bind with rotavirus particles specifically. Plaque reduction neutralization test and MTT analysis results indicated that 3 of the peptides can inhibit rotavirus infecting in vitro. A peptide which sequence is QSNPIHIITNTRNHP showed the best efficiency—93% neutralization infectivity. Two other peptides，A and B，showed 40% and 50% neutralization infectivity respectively. Amino sequence analysis results indicate the 3 peptides containing 2 conserved motifs: SNPIHII and NIP. No putative trypsin hydrolysis site was found in C peptide，however，4 and 3 potential sites were found in A and B peptides respectively. Using trypsin inhibitor，both A and B peptides showed the similar antiviral effect as that of C peptide. It suggests that the intactness of the 2 conserved motifs play an important role in counteracting virus infection. According to the results of this study，peptide C is hopeful to be exploited as an antiviral peptide drug.