Coordination Mechanism and Antioxidant Activity of L-hydroxyproline Zinc(Ⅱ)

Abstract:

The coordination compound of L-hydroxyproline(Hyp)-Zn(Ⅱ) was synthesized with Hyp and zinc sulfate as raw materials in water medium, coordination Synthesizing Mechanism and Antioxidant Activity of Hyp-Zn(Ⅱ)coordination compound has been researched. Compared with Hyp, the infrared spectrogram of Hyp-Zn(Ⅱ) coordination compound emerge a new absorption peak at 1100cm^－1. Conclusion could be obtained that there exists a coordination effect between Hyp and ZnSO₄; TG and DSC curve of Hyp and Hyp-Zn(Ⅱ) coordination compound were analysed. Compared with Hyp, the peak of Hyp-Zn(Ⅱ) disappear at 290℃ and 375℃. This phenomenon confirmed the front conclusion; At the NMR graph of Hyp-Zn(Ⅱ)coordination compound, the disappearance of the α-carboxyl-hydrogen and α-hydroxyl- hydrogen's peak at 3.5～3.9ppm could indicate that combination's position of Hyp isα-carboxyl and α-hydroxyl; Structure of Hyp-Zn(Ⅱ) coordination compound were exosyndrome by the Atomic Force microscopy. It is showed that centr-atom Zn(Ⅱ) was surrounded by several Hyp at Hyp-Zn(Ⅱ) coordination compound's phase diagram; The proportion of Hyp-Zn(Ⅱ) coordination compound was determined by dialysis experiment, the proportion is 4∶1; Above-mentioned determination and exosyndrome indicated that the molecular formula of Hyp-Zn(Ⅱ) coordination compound is Zn(Hyp)₄·H₂O. The results indicated that the Hyp-Zn(Ⅱ) coordination compound can inhibit hydroxyl free radicals of Zn (Ⅱ), and the Percentage of Inhibition is 75.5% ;the total antioxidant activities of Hyp-Zn(Ⅱ) coordination compound is 80.167u/mL, the anti-superoxide activities of Hyp-Zn(Ⅱ) coordination compound is 53.19u/mL.