Cloning and Sequence Analysis of SOCS-2 Gene in Pig

Abstract:

Total RNA was isolated from kidney of BaMei pig，a local strain of Chinese pig, and then the cDNA sequence of SOCS-2 gene was cloned by RT-PCR (GenBank accepted number is EF121242). Then the cloned SOCS-2 gene was inserted into PMD19-T vector by T/A cloning, transformed into DH-5α, tested by PCR and sequenced. The data show that the homology of the cloned porcine SOCS-2, including 822 bp,is more than 93% and that of the deduced amino acid sequence is 89% when compared with human, rat and mice. And the molecular weight of SOCS-2 protein is about 22.25kD and PI is 8.03. The cloning of SOCS-2 gene is useful for the further research on the molecular mechanism by which regulating growth and development of organism.