Application of Spindle-view in the Enucleation Porcine of Oocytes

Abstract:

The Spindle-view，a specialized instrument for observing spindle image，was applied to observe the meiotic spindles of vitro matured porcine oocytes at 36、42、44、48h，and enucleation from porcine, comparing to the previously methods (McGrath-Solter's method and two-step-squeezing method) in the enucleated. The results showed that:①there was no noticeable differences at vicinity of spindle images and 1st polar body among in vitro matured porcine oocytes at 40～48h under the instrument；②Spindle-view is suitable for the observation of meiotic spindles of matured oocytes and enucleation from porcine; the modified Spindle-view method for enucleation is significantly better than McGrath-Solter's method and two-step-squeezing method in the enucleated rates (95.5%,42.1%,74.2%,P<0.01)of absolutely removing nuclei matter;③the spindle images could be used to monitor the oocyte qualities.