Construction and Cultivation of Genetically-engineered Strain to Improve Geldanamycin Production

Abstract:

To improve the production of geldanamycin in Streptomyces hygroscopicus 17997, gene disruption was done to delete the naphthalenic AHBA genes (shnSOP), encoding the products that share the common biosynthetic substrates with geldanamycin. The resulting mutant strain (DSOP) was cultivated on a solid medium and the amount of spores collected from the plates was calculated from 5 to 14 days and the yield of geldanamycin was measured by HPLC. The geldanamycin production of the DSOP strain increased by 185% comparing with that of the parent strain. On solid medium, the DSOP strain underwent 2 cycles of sporulation and the growth of the second sporulation had the highest geldanamycin production.