Exogenous Leptin Inhibits Differentiation via MAPK in Swine Skeletal Myoblasts

Abstract:

We determined whether body-weight regulator, leptin, could influence differentiation of swine skeletal myoblasts. Myoblast occured in non-leptin and leptin formed in different concentrations (10~1000 ng/mL) for various duration (12~60 h). The morphologic assay demonstrated that leptin significantly decreased the formation of myotubes and myogenic index. In addition, biochemical analysis showed that leptin inhibited Creatine Kinase (CK) activity, expressions of myogenin and mitogen activated protein kinase (MAPK). As conclusion, exogenous leptin could inhibit differentiation via MAPK in swine skeletal myoblasts and this suggests that leptin be an important mediator in the regulation of muscle-cell differentiation.