Effect of Tumstatin Anti-tumor Peptide on Proliferation and Apoptosis of Hepatocarcinoma Cell

Abstract:

In this study, the basic sequences of 19peptide were synthesized and inserted into vector pTYB2. After being expressed and purified, the soluble 19peptide was obtained. The inhibiting effect on hepatocarcinoma cell was selected by 3-[4, 5-dimehyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay and cell growth curve. The apoptosis index was observed using TdT-mediated dUTP nick end labeling (TUNEL), flow cytometry and transmission electron microscope (TEM). Its anti-tumor activity in vivo was verified in H22 ascitic fluid transfevent hepatocarcinoma of mice. 3-[4, 5-dimehyl-2-thiazolyl]-2, 5-di- phenyl-2H-tetrazolium bromide (MTT) assay and cell growth curve showed cell viability decrease with the 19peptide.Survival hepatocarcinoma cell decreased with time. In the treatment group, obvious change of apoptosis, the appearance of sub-G1 phase and dyed brown cells were seen by transmission electron microscope (TEM), flow cytometry and TdT-mediated dUTP nick end labeling (TUNEL), The inhibition rate of mouse H22 ascitic fluid transfevent hepatocarcinoma growth was 48.46%. As a whole, 19peptide could directly inhibit hepatocarcinoma cell proliferation and promote hepatocarcinoma cell apoptosis.