Rapid Separation of Antiserum by Surface Modified Fe3O4 Nano-particles

Abstract:

The nano-particles’ surface were modified by silane reagent Si(OC2H5)3C3H6NH2. Bovine serum albumin (BSA) was coupled by a series of glutaraldehyde concentration. Under optimal reaction conditions the maximum amount of immobilized BSA was 140 mg/mg Fe3O4, when glutaraldehyde concentration was above 10%. After immersing in anti-BSA rabbit serum and regenerating by Gly-HCl buffer, the purity of IgG by super-paramagnetic iron oxide (SIPO) nano-particles was characterized by UV-spectrometer, SDS-PAGE and immuno-diffuse. Results showed that subtotal separation was achieved at 10 min, 15mg SPIO in 1ml serum. The purity was much higher than that with the traditional (NH4)2SO4 method. The activity of special protein probe maintained above 78% after 5 consecutive uses.