Wenlu Zeng
1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China; 2 College of Environmental Science and Engineering, Tianjin Key Laboratory of Environmental Remediation andFeifei Zhao
1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, ChinaZhaogen Cao
1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, ChinaBinggen Ru
1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, Chinathe National Science Foundation for Post-doctoral Scientists of China (No. 2003033079).
To implement high cell density cultivation of transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-I gene, and its applications in heavy metal wastewater, we optimized the medium components. We used response surface method, a useful tool for multi-factor process optimization, through a three- step experiment design: full factor design, the steepest climbing and central composite design. The selected three factors were NaHCO3, NaNO3, and initial pH. The optimized medium contained 1.696 g/L NaHCO3 and 8.57 g/L NaNO3, at initial pH 8.57. The optimized medium was validated in 2-L and 20-L air lift photo-bioreactor, respectively. The maximum cell concentration reached 4.16 g/L for 2-L bioreactor, and 3.12 g/L for 20-L bioreactor.
曾文炉,赵飞飞,曹照根,茹炳根. 利用响应面方法优化转小鼠金属硫蛋白-I基因聚球藻7002的培养基成分[J]. Chinese Journal of Biotechnology, 2008, 24(1): 130-136
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