Cloning and Characterization of the Chloramphenicol Resistant Gene of Serratia marcescens Strain KMR-3

Abstract:

Chloramphenicol-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. The protein belonged to PRK10473 protein, and it showed 92% similarity to drug resistance transporter, Bcr/CflA subfamily of Serratia proteamaculans 568. Regulation elements including promoter, terminator, Shine–Dalgarno (SD) sequence and transcription start site also were identified.