Heterologous Expression and Characterization of L-Amino Acid Deaminase from Proteus mirabilis in Escherichia coli

Abstract:

We expressed an L-amino acid deaminase (Pma) from Proteus mirabilis in Escherichia coli and characterized the kinetics of phenylpyruvic acid production. P. mirabilis Pma was well expressed in E. coli in an active state and was found to be associated with membranes. The association of Pma with cellular membranes is likely to be necessary for its enzymatic activity.