Rapid establishment of suspension cell lines in japonica rice

Abstract:

With Jingkang No.5 (PiA), calli of the PiA induced for 10?15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7?10 days, and the cells cultured for 30?120 days had the best differentiation ability (57.1%) and regeneration ability (20%). This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation.