Cloning, expression of the porcine Estrogen-related receptor a gene and its effect on lipid accumulation in mature adipocytes

Abstract:

Estrogen-related receptor a (ERRa) is an orphan nuclear receptor and functions as a key regulator of energy metabolism in high energy demand tissues. However, its role in white adipose tissue is largely unknown. In this study, we aim to clone the ORF sequence of pig ERRα with touch down-PCR, analyze the expression pattern of ERRa protein and its subcellular localization with Western blotting and cell immunofluorescence method respectively, and identify the effect of ERRα on lipid accumulation in mature porcine adipocytes with its specific inhibitor XCT790. The results showed that the ERRα ORF sequence is 1269 bp (GenBank Accession No. FJ446485, not published), and encode 422 amino acids. The homologies of ERRα nucleotide and amino acids sequences are high in different species. ERRα protein is highly expressed in pig white adipose tissue, kidney and heart, while remarkably lower in spleen. Cell immunofluorescence results indicated that ERRα protein distribute widely in adipocytes nucleus and cytoplasm. XCT790 significantly inhibited the expression of ERRα and lipid accumulation in porcine mature adipocyte. This study will provide new target and theoretical reference for fat deposition control.