Key Scientific and Technological Project of Jiangxi Province (No. 20061B0260301), Educational Commission of Jiangxi Province (No. GJJ09057), Innovation Project in Graduate Education of Jiangxi Province (No. YC08A018).
The grass carp (Ctenopharyngodon idella) collectin gene was cloned from mixed liver and kidney cDNA library. The full length sequence of grass carp collectin was 1128 bp, contained a 5? untranslated region of 229 bp and a 3? untranslated region of 104 bp. The open reading frame of grass carp collectin was 795 bp which could code a 264 amino acids polypeptide, including a terminal codon. Phylogenetic analyses showed that grass carp collectin shared the highest homology with that of zebrafish (Danio rerio). To understand the function of grass carp collectin, we expressed and purified the recombinant protein (PCRD) that comprised carbohydrate recognition domain (CRD). Agglutination of Aeromonas hydrophila and Staphylococcus aureus etc. and sugars inhibition experiments showed that: galactose, glucose, mannose and maltose could inhibit the agglutination of Aeromonas hydrophila. Maltose could lower the agglutination of Staphylococcus aureus, whereas peptidoglycan and glucose inhibited it well. In addition, the activity of grass carp collectin could not dependent on Ca2+.
李琴,马梅生,胡成钰. 草鱼胶原凝集素基因的克隆及其功能分析[J]. Chinese Journal of Biotechnology, 2010, 26(1): 22-27
Copy® 2024 All Rights Reserved