Hongwen Chen
Key Laboratory of Industrial Biotechnology, Fujian Province, Huaqiao University, Xiamen 361021, ChinaJinfeng Nie
Key Laboratory of Industrial Biotechnology, Fujian Province, Huaqiao University, Xiamen 361021, ChinaGuo Chen
Key Laboratory of Industrial Biotechnology, Fujian Province, Huaqiao University, Xiamen 361021, ChinaBaishan Fang
Key Laboratory of Industrial Biotechnology, Fujian Province, Huaqiao University, Xiamen 361021, ChinaNational High Technology Research and Development Program of China (863 Program) (No. 2006AA020103), National Natural Science Foundation of China (No. 20676048), Natural Science Foundation of Fujian Province (No. D0810015), New Century Excellent Talents i
The kinetic mechanisms of two key enzymes in the biotransformation of glycerol to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae, glycerol dehydrogenase (GDH) and 1,3-propanediol oxidoreductase (PDOR), was characterized. Kinetics on initial velocity and product inhibition revealed that GDH and PDOR follow an ordered Bi-Bi sequential mechanism. Kinetic models for GDH and PDOR showed that the oxidation reaction catalyzed by GDH was the rate-limiting step in coupled enzymatic reaction when the GDH/PDOR was 1:1, and the NAD+ was the main form of coenzyme in the reaction. Knowledge about the kinetic mechanisms will be helpful to understand how these enzymes is regulated, which will be useful for further enzyme catalysis and metabolic engineering studies.
陈宏文,聂金峰,陈国,方柏山. 克雷伯杆菌甘油脱氢酶和1,3-丙二醇氧化还原酶的动力学机制[J]. Chinese Journal of Biotechnology, 2010, 26(2): 177-182
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