National Natural Science Foundation of China (No. 20976051), Ministry of Education of China (No. 20090074110013), Fundamental Research Funds for the Central Universities, Shanghai Leading Academic Discipline Project (No. B505), China National Special Fund
The massive water and steam are consumed in the production of cellulose ethanol, which correspondingly results in the significant increase of energy cost, waster water discharge and production cost as well. In this study, the process strategy under extremely low water usage and high solids loading of corn stover was investigated experimentally and computationally. The novel pretreatment technology with zero waste water discharge was developed; in which a unique biodetoxification method using a kerosene fungus strain Amorphotheca resinae ZN1 to degrade the lignocellulose derived inhibitors was applied. With high solids loading of pretreated corn stover, high ethanol titer was achieved in the simultaneous saccharification and fermentation process, and the scale-up principles were studied. Furthermore, the flowsheet simulation of the whole process was carried out with the Aspen plus based physical database, and the integrated process developed was tested in the biorefinery mini-plant. Finally, the core technologies were applied in the cellulose ethanol demonstration plant, which paved a way for the establishment of an energy saving and environment friendly technology of lignocellulose biotransformation with industry application potential.
张建,楚德强,于占春,张小希,邓红波,王修胜,朱智楠,张怀庆,戴干策,鲍杰. 低水用量约束条件下的高固体含量纤维乙醇生物加工技术策略[J]. Chinese Journal of Biotechnology, 2010, 26(7): 950-959
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