Jing Zhang
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaJingwen Zhou
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaLiming Liu
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaJie Liu
Jiangsu Jiangshan Pharmaceutical Co., Ltd., Jingjiang 214500, ChinaKejie Chen
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaGuocheng Du
Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaJian Chen
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Biological Systems and Bio-processing Laboratory, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaNational Science Fund for Distinguished Young Scholars (No. 20625619), National Basic Research and Development Program of China (973 Program) (No. 2007CB 714306), Innovative Research Team for University of Jiangsu Province, National High Technology Resear
The aim of this study was to improve the 2-keto-L-gulonic acid (2-KLG) production efficiency by Ketogulonicigenium vulgare and Bacillus megaterium by using multi-stage pH control strategy. The effect of pH on the cell growths and 2-KLG production showed that the optimum pH for K. vulgare and B. megaterium cell growth were 6.0 and 8.0, respectively, while the optimum pH for 2-KLG production was 7.0. Based on the above results, we developed a three-stage pH control strategy: the pH was kept at 8.0 during the first 8 h, then decreased to 6.0 for the following 12 h, and maintained at 7.0 to the end of fermentation. With this strategy, the titer, productivity of 2-KLG and L-sorbose consumption rate were achieved at 77.3 g/L, 1.38 g/(L·h) and 1.42 g/(L·h), respectively, which were 9.7%, 33.2% and 25.7% higher than the corresponding values of the single pH (pH 7.0) control model.
张静,周景文,刘立明,刘杰,陈克杰,堵国成,陈坚. 分阶段pH调控提高2-酮基-L-古龙酸生产[J]. Chinese Journal of Biotechnology, 2010, 26(9): 1263-1268
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