Bing Xu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, ChinaMin Jiang
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, ChinaJiangfeng Ma
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, ChinaShuwen Liu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, ChinaGuwei Hou
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, ChinaShanshan Sui
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, ChinaNational Basic Research and Development Program of China (973 Program) (No. 2011CB707405), National Natural Science Foundation of China (No. 21076105), State Key Laboratory of Materials-Oriented Chemical Engineering, “Qinglan Project” of Jiangsu Province, “The Six Talent Summit” of Jiangsu Province (No. 06-A-047).
The possibility of reusing Escherichia coli cells from the broth for succinic acid production was investigated. Using succinic acid yield and productivity as criterion, we investigated the effects of cell concentration, initial glucose concentration, different neutralizers on the bioconversion. The results revealed that E. coli could convert glucose to succinic acid in a water solution of glucose and a neutralizer. According to the results, the optimal condition was as follows: the cell concentration was 50 (OD600), glucose concentration was 40 g/L and neutralizer was MgCO3. Under the optimum conditions, we carried out the consecutive batch bioconversion in 7 L fermenter. Succinic acid yield reached 91% with the productivity of 3.22 g/(L·h) for the first conversion. For the second conversion, succinic acid yield reached 86% with productivity of 2.04 g/(L·h). Furthermore, we achieved a high mass yield above 83% with the productivity of 1.82 g/(L·h) for the third bioconversion.
徐冰,姜岷,马江锋,刘树文,侯顾伟,隋姗姗. 循环利用重组大肠杆菌细胞转化合成丁二酸[J]. Chinese Journal of Biotechnology, 2010, 26(11): 1526-1531
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