Tongwei Su
1 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaBin Bao
1 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2 Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, ChinaTing Yan
1 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaChaoyan Zhang
1 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 3 Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, ChinaYongshi Bu
1 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaWenhui Wu
2 Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China; 3 Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, ChinaNational High Technology Research and Development Program of China (863 Program) (No. 2011AA09070109).
Response surface methodology was applied to optimize the fermentation conditions of FGFC1 (Fungi fibrinolytic compound 1). On the basis of single factor tests, response surface analysis was designed by Design-Expert, and the effects of culture time, ornithine hydrochloride addition and culture temperature on the yield of FGFC1 were studied, the predicted value and measured value were also contrasted. The results show the optimal culture conditions as follows: the culture time is 7 d, ornithine hydrochloride addition is 0.5% (M/V), culture temperature is 28 °C. Under these conditions, the yield of FGFC1 is 1 978.33mg/L, which is consistent with the predicted value. It shows that the experiment is effective.
苏同伟,包斌,严婷,张朝燕,卜永士,吴文惠. 响应面法优化海洋微生物发酵产生纤溶化合物的培养条件[J]. Chinese Journal of Biotechnology, 2013, 29(6): 857-861
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