Health and the Welfare industry Research Special Funds (No. 200702008), Key Projects in the National Science and Technology Pillar Program during the Twelfth Five-Year Plan Period (Nos. 2011BA110B00, 2011BA110B05).
To compare two enrichment and preservation methods of urinary proteins, stored in polyvinylidene difluoride (PVDF) membrane (Urimem) or direct freezing, we examined the differences between the two methods in time, space, costs of supplies and electricity, degree of protein degradation and convenience of the sample handling. The urimem method is superior in the storage space, the cost of electricity and the clinical convenience compared to the direct freezing method. However, the direct freezing method is superior in the time and the cost of supplies to the urimem method. The enrichment and preservation of urinary proteins using urimem have more cost-effective benefits compared to those of the direct freezing method.
王明铭,刘雪姣,贾露露,孙颖,高友鹤,李明喜. 尿蛋白膜保存法与直接冻存法的成本效益分析[J]. Chinese Journal of Biotechnology, 2014, 30(7): 1128-1133
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