Kun Sun
1 College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, ChinaFan Yang
1 College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, ChinaYingjun Kong
2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, ChinaJiyao Kang
2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, ChinaWei Cao
1 College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, ChinaXiaoyan Yang
1 College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, ChinaShenghua Zha
2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, ChinaGuifeng Zhang
2 State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, ChinaMinglin Wang
1 College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, ChinaNational High Technology Research and Development Program of China (863 Program) (No. 2014AA022109).
A method for quantitation of collagen was established by detecting marker peptide with high performance liquid chromatography-mass spectrometry (HPLC-MS). Theoretical marker peptides were selected by sequence comparison. Bovine collagen type I was digested with trypsin. Marker peptides typical for collagen type I were identified with HPLC-MS. The relationship between the abundance of marker peptides and collagen concentration was established. The results show that GEAGPSGPAGPTGAR and the other 5 peptides showed high resolution during chromatographic separation and high signal intensity during MS analysis. Peptide signal intensity and collagen concentration showed a good linear relationship in the range from 0.1 to 3 mg/mL. Bovine tendon and collagen sponge were used as actual samples and collagen contents were determined as 90.2% and 93.4% respectively. Quantitation of marker peptides of collagen was a feasible method to identify and quantify collagens in medical device research and development.
孙坤,杨帆,孔英俊,康跻耀,曹玮,杨小雁,査圣华,张贵锋,王明林. 基于生物质谱的胶原蛋白定量检测方法[J]. Chinese Journal of Biotechnology, 2015, 31(11): 1660-1668
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