National Natural Science Foundation of China (Nos. 31571374, 31622047).
In the past 4 years, CRISPR/Cas9-mediated genome editing becomes the revolutionary tool in life sciences. This tool enables us to edit plant genes with unprecedented throughput, scalability, speed and low cost. In addition to targeted knock-in and knock-out applications, CRISPR/Cas9 also provides an efficient platform for targeted gene activation and suppression. At the same time, accuracy, capacity and efficiency of CRISPR/Cas9 genome editing have been improved for sophisticated genetic manipulation. Furthermore, the genome editing toolbox is expanded by new technologies like CRISPR/Cpf1-mediated genome editing and single base editing. Owing to these recent progresses, CRISPR technology is close to the dream tool for plant sciences and will accelerate the crop genetic improvement through precise genome editing.
李红,谢卡斌. 植物CRISPR基因组编辑技术的新进展[J]. Chinese Journal of Biotechnology, 2017, 33(10): 1700-1711
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