National Natural Science Foundation of China (Nos. 31070089, 31170078, J1103514), National High Technology Research and Development Program of China (863 Program) (Nos. 2011AA02A204, 2013AA065805).
Enzyme is an efficient and green biocatalyst, and widely used in many areas. Immobilized enzyme is superior to its free form in a variety of properties. Enzyme immobilization studies started in the 1970s in China. Till now, immobilized enzymes are widely applied in the fields of food, medical, energy, environmental management, among others. However, there are still some defects such as no universal method and the high cost. Therefore, based on the relatively mature traditional immobilization technologies, efforts have been made to innovate immobilization technologies. As a result, many new immobilization technologies focusing on new carriers and methods are continuously generated. Coupling with more than ten years’ study on enzyme immobilization, we present here recent development and application of new immobilization technologies, as well as suggestions to future development of immobilization technology.
柯彩霞,范艳利,苏枫,徐莉,闫云君. 酶的固定化技术最新研究进展[J]. Chinese Journal of Biotechnology, 2018, 34(2): 188-203
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