Ning Ding
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaChao Li
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaLi Bai
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaMeijin Guo
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2 Shanghai Biological Manufacturing Technology Innovation Center, Shanghai 200237, ChinaYingping Zhuang
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2 Shanghai Biological Manufacturing Technology Innovation Center, Shanghai 200237, ChinaSiliang Zhang
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaNational High Technology Research and Development Program of China (863 Program) (No. 2012AA021201).
Bioreactors have been central in monoclonal antibodies and vaccines manufacturing by mammalian cells in suspension culture. Numerical simulation of five impeller combinations in a stirred bioreactor was conducted, and characteristics of velocity vectors, distributions of gas hold-up, distributions of shear rate in the bioreactor using 5 impeller combinations were numerically elucidated. In addition, genetically engineered CHO cells were cultivated in bioreactor installed with 5 different impeller combinations in fed-batch culture mode. The cell growth and antibody level were directly related to the maximum shear rate in the bioreactor, and the highest viable cell density and the peak antibody level were achieved in FBMI3 impeller combination, indicating that CHO cells are sensitive to shear force produced by impeller movement when cells were cultivated in bioreactor at large scale, and the maximum shear rate would play key roles in scaling-up of bioreactor at industrial scale.
丁宁,李超,白力,郭美锦,庄英萍,张嗣良. 机械搅拌式动物细胞反应器不同桨型组合的CFD数值模拟与优化[J]. Chinese Journal of Biotechnology, 2020, 36(6): 1209-1215
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