Jiancai Wang
1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;2 Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, Shandong, China;3 Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Jinan 250100, Shandong, ChinaXinghua Liu
1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;2 Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, Shandong, China;3 Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Jinan 250100, Shandong, ChinaHuaizhong Wang
1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;2 Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, Shandong, China;3 Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Jinan 250100, Shandong, ChinaHongmei Hu
1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;2 Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, Shandong, China;3 Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Jinan 250100, Shandong, ChinaLili Pang
4 College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong, ChinaBaohua Huang
1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;2 Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, Shandong, China;3 Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Jinan 250100, Shandong, ChinaRongsheng Zhu
1 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China;2 Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, Shandong, China;3 Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Jinan 250100, Shandong, ChinaChina Postdoctoral Science Foundation (No. 2017M612316), Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (No. CXGC2016B13), the Key R & D Project of Shandong Province, China (No. 2017CXGC0309).
Adding biological passivation agent during composting is one of the most effective ways to reduce the toxicity of heavy metals in contaminated livestock manure. To further improve biological passivation, we obtained a strain with high-heavy metal compounds tolerance to passivate heavy-metal contaminated manure and to characterize heavy-metal biosorption. High-tolerance microorganisms for lead and cadmium were isolated and screened from swine manure composting samples. The strain was identified by its morphology and molecular biology. After the influence of different pH, temperature and salt concentrations on growth of the strain were investigated, the optimal growth conditions were obtained for further analysis of its biosorption characteristics of lead and cadmium. The bacterium with tolerance to lead and cadmium termed SC19 was obtained, whose lead resistance was 600 mg/L and cadmium resistance was 120 mg/L. The isolate was further identified as Cedecea sp., and then its optimum pH was 7.0, temperature was 37 °C, and salt concentration was 0.5%. Lead removal was highest after 30 min of adsorption by the SC19 strain cultured for the stationary phase 36 h, and the maximum removal rate and biosorption capacity of lead were 60.7% and 329.13 mg/g, respectively. Meanwhile, cadmium removal was highest after 30 min of adsorption by the strain cultured for the logarithmic phase 8 h, and the maximum removal rate and biosorption capacity of cadmium were 51.0% and 126.19 mg/g, respectively. Fourier Transform InfraRed (FT-IR) results revealed that the biosorption process mainly happened on the surface of SC19 cell and many active groups on the cell surface could chelate the Pb2+ and Cd2+. By comprehensive comparison, it was showed that strain SC19 shared a certain capacity of Pb2+ and Cd2+ biosorption, and the bacterium provided precious microbial germplasm resources for biological passivation of heavy metal contaminated manure.
王建才,刘兴华,王怀中,呼红梅,庞丽丽,黄保华,朱荣生. 耐铅镉菌株的分离鉴定及其吸附能力[J]. Chinese Journal of Biotechnology, 2020, 36(8): 1600-1609
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