National Key Research and Development Program of China (Nos. 2018YFC1602900, 2018YFC1602500).
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
方水琴,刘程,马俊飞,闫帅帅,许东坡,刘箐. 小鼠杂交瘤单克隆抗体快速制备技术研究进展[J]. Chinese Journal of Biotechnology, 2021, 37(7): 2293-2306
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