Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma
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National Key Research and Development Program of China (Nos. 2018YFC1602900, 2018YFC1602500).

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    Abstract:

    Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.

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方水琴,刘程,马俊飞,闫帅帅,许东坡,刘箐. 小鼠杂交瘤单克隆抗体快速制备技术研究进展[J]. Chinese Journal of Biotechnology, 2021, 37(7): 2293-2306

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  • Received:May 16,2020
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  • Online: July 27,2021
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