Minfang You
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, ChinaYuanyu Qin
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, ChinaYiyu Zhang
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, ChinaChaomei Liao
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, ChinaGuanghui Tan
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, ChinaJiezhang Li
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, ChinaWangui Li
2 The Agriculture and Rural Affairs Bureau of Liupanshui, Liupanshui 553001, Guizhou, ChinaNational Natural Science Foundation of China (No. 31760663), Guizhou Province Science and Technology Talents Subsidy Project, China (No. [2017]5788), Guizhou Province Thousand-level Innovative Talent Project, China (No. 701030174401).
Inositol 1,4,5-trisphosphate receptor 1 (ITPR1) is an important intracellular channel for releasing Ca2+. In order to investigate the effects of the ITPR1 overexpression on Ca2+ concentration and lipid content in duck uterine epithelial cells and its effects on calcium transport-related genes, the structural domain of ITPR1 gene of duck was cloned into an eukaryotic expression vector and transfected into duck uterine epithelial cells. The overexpression of the ITPR1 gene, the concentration of Ca2+, the lipid content, and the expression of other 6 calcium transport-related genes was determined. The results showed that the concentration of Ca2+ in uterine epithelial cells was significantly reduced after transfection (P<0.05), the triglyceride content was significantly increased (P<0.01), and the high-density lipoprotein content was significantly decreased (P<0.01). The correlation analysis results showed that the overexpression of the C-terminal half of the ITPR1 gene was significantly positively correlated with the total cholesterol content (P<0.01), which was significantly positively correlated with the low-density lipoprotein content (P<0.05). The overexpression of the N-terminal half of the ITPR1 gene was significantly positively correlated with the triglyceride content (P<0.01), which was significantly negatively correlated with the concentration of Ca2+ (P<0.05). RT-qPCR results of 6 calcium transport-related genes showed that the overexpression of the C-terminal half of the ITPR1 gene significantly inhibited the expression of the IP3R2, VDAC2 and CAV1 genes, and the overexpression of the N-terminal half of the ITPR1 gene significantly promoted the expression of the IP3R3 and CACNA2D1 genes. In conclusion, the ITPR1 gene overexpression can promote Ca2+ release in duck uterus epithelial cells, promote the synthesis of triglyceride, low-density lipoprotein and cholesterol, and inhibit the production of high-density lipoprotein, and the ITPR1 gene overexpression affected the expression of all 6 calcium transport-related genes.
游敏芳,覃媛钰,张依裕,廖朝美,谭光辉,李杰章,李万贵. ITPR1基因过表达对鸭子宫上皮细胞Ca2+浓度、脂质含量及钙转运相关基因的调控效应[J]. Chinese Journal of Biotechnology, 2021, 37(7): 2443-2452
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