Advances in synthetic biology of CO2 fixation by heterotrophic microorganisms

Abstract:

Human activities increase the concentration of atmospheric carbon dioxide (CO₂),which leads to global climate warming.Microbial CO₂ fixation is a promising green approach for carbon neutral.In contrast to autotrophic microorganisms,heterotrophic microorganisms are characterized by fast growth and ease of genetic modification,but the efficiency of CO₂ fixation is still limited.In the past decade,synthetic biology-based enhancement of heterotrophic CO₂ fixation has drawn wide attention,including the optimization of energy supply,modification of carboxylation pathway,and heterotrophic microorganisms-based indirect CO₂ fixation.This review focuses on the research progress in CO₂ fixation by heterotrophic microorganisms,which is expected to serve as a reference for peaking CO₂ emission and achieving carbon neutral by microbial CO₂ fixation.