Optimization of retinin expression and the application with wax emulsion in nanocoatings
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    Abstract:

    Anti-reflective nanocoatings that mimic the eyes of fruit flies are biodegradable materials with great market potential for a variety of optical devices that require anti-reflective properties. Microbial expression of retinin provides a new idea for the preparation of nanocoatings under mild conditions compared to physicochemical methods. However, the current expression level of retinin, the key to anti-reflective coating, is low and difficult to meet mass production. In this study, we analyzed and screened the best expression hosts for Drosophila-derived retinin protein, and optimized its expression. Chinese hamster ovary (CHO) cells were identified as the efficient expression host of retinin, and purified retinin protein was obtained. At the same time, the preparation method of lanolin nanoemulsion was explored, and the best anti-reflective ability of the nano-coating was determined when the ratio of specific concentration of retinin protein and wax emulsion was 16:4, the pH of the nano-coating formation system was 7.0, and the temperature was 30℃. The enhanced antireflective ability and reduced production cost of artificial antireflective nanocoatings by determining the composition of nanocoatings and optimizing the concentration, pH and temperature of system components may facilitate future application of artificial green degradable antireflective coatings.

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刘宇晴,夏媛媛,沈微,杨海泉,陈献忠. 视黄素蛋白的表达优化及其与蜡乳液在纳米涂层中的应用[J]. Chinese Journal of Biotechnology, 2023, 39(10): 4258-4274

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History
  • Received:February 19,2023
  • Adopted:May 23,2023
  • Online: October 17,2023
  • Published: October 25,2023
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