This article summarized the reviews and research articles published in Chinese Journal of Biotechnology in the field of biomanufacturing in 2021.The article covered major chassis cells such as Escherichia coli,Bacillus subtilis,Corynebacterium glutamicum,Saccharomyces cerevisiae,filamentous fungi,non-model bacteria and non-conventional yeasts.Moreover,this article summarized the advances in the production of amino acids,organic acids,vitamins,higher alcohols,natural compounds (terpenoids,flavonoids,alkaloids),antibiotics,enzymes and enzyme-catalyzed products,biopolymers,as well as the utilization of biomass and one-carbon materials.The key technologies used in the construction of cell factories,such as regulation,evolution,and high-throughput screening,were also included.This article may help the readers better understand the R & D trend in biomanufacturing driven by engineered microbes.
李寅.合成生物制造[J].Chinese Journal of Biotechnology,2022,38(4):1267-1294 Export BibTexEndNote
Unnatural amino acids are widely used in medicine,pesticide,material,and other industries and the green and efficient synthesis has attracted a lot of attention.In recent years,with the rapid development of synthetic biology,microbial cell factories have become a promising means for biosynthesis of unnatural amino acids.This study reviewed the construction and application of microbial cell factories for unnatural amino acid,including the synthetic pathway reconstruction,design/modification of key enzymes and their coordinated regulation with precursors,blocking of competitive alternative pathways,and construction of cofactor circulation systems.Meanwhile,on the basis of the new principles for designing the microbial cell factories,new biosynthetic pathways adapted to cells and the production environment,as well as new biomanufacturing system established based on cell adaptive evolution and intelligent fermentation regulation,we looked forward to the further construction and application of microbial cell factories for industrial bio-production.
汤晓玲，张慧敏，柳志强，郑裕国.非天然氨基酸细胞工厂的构建与应用[J].Chinese Journal of Biotechnology,2022,38(4):1295-1306 Export BibTexEndNote
Tetrapyrrole compounds are a class of compounds with important functions.They exist in living organisms and have been widely used in agriculture,food,medicine,and other fields.The cumbersome process and high cost of chemical synthesis,as well as the shortcomings of unstable quality of animal and plant extraction methods,greatly hampered the industrial production and applications of tetrapyrrole compounds.In recent years,the rapid development of synthetic biology has provided new tools for microorganisms to efficiently synthesize tetrapyrrole compounds from renewable biomass resources.This article summarizes various strategies for the biosynthesis of tetrapyrrole compounds,discusses methods to improve its biosynthesis efficiency and future prospects,with the aim to facilitate the research on biosynthesis of tetrapyrrole compounds.
潘斐，严一凡，朱逸凡，胡毅，续晓琪，徐铮，王瑞，李莎，徐虹，罗正山.四吡咯化合物生物合成研究进展[J].Chinese Journal of Biotechnology,2022,38(4):1307-1321 Export BibTexEndNote