空间转录组学技术及其在植物研究中的应用

Spatial transcriptomics techniques and its applications in plant research

摘要:

植物细胞基因表达的异质性是造成组织功能差异的关键因素。近年来，利用空间转录组测序技术研究植物特定生物学问题已取得较大突破，现已成功应用于细胞发育、细胞鉴定和抗逆应激等方面。为探究空间转录组测序技术在植物中的应用，本文对空间转录组测序技术的发展、在植物中的应用以及未来研究方向3个方面进行综述，系统阐述了空间转录组测序技术的发展进程，重点分析其在植物细胞生长和分化、植物细胞鉴定、抗逆应激的应用中所取得的进展。此外，总结空间转录组测序技术在植物应用中存在的挑战，提出未来在植物研究中的方向以及结合其他组学技术的优势，以期利用空间转录组测序技术解决更多植物领域的科学问题。

Abstract:

The heterogeneity of gene expression in plant cells plays a crucial role in determining the functional differences among tissues. Recent advancements in spatial transcriptome (ST) technology have significantly contributed to the study of specific biological questions in plants. This technology has been successfully applied to examine cell development, identification, and stress resistance. This review aims to explore the application of ST technology in plants by reviewing three aspects: the development of ST technology, its current application in plants, and future research directions. The review provides a systematic description of the development process of ST technology, with a focus on analyzing its progress in studying plant cell growth and differentiation, plant cell identification, and stress resistance. In addition, the challenges faced by ST technology in plant applications are summarized, along with proposed future directions for plant research, including the advantages of combining other omics technologies with ST technology to tackle scientific challenges in the field of plants.