生产双链RNA工程菌的研究进展

Research progress in the engineering strains for producing double-stranded RNA

摘要:

保障粮食安全需要新型绿色农药。双链RNA (double-stranded RNA,dsRNA)农药通过外源添加特异性靶向病虫害的dsRNA来触发RNA干扰，抑制病原菌或害虫的关键基因表达，从而实现对特定病虫害的有效控制。双链RNA农药是一种环境友好型的农药，具有较强的特异性和高效的基因沉默能力，但目前还存在生产成本高等问题。利用工程菌株生产dsRNA是一种可行的策略，然而目前还没有具有经济效益的dsRNA生产工程菌株出现。本文综述了利用微生物生产dsRNA的研究进展和生产策略，为dsRNA生产提供了参考。

关键词:RNA干扰;双链RNA;工程菌株;底盘

Abstract:

Ensuring food security requires new green pesticides. Double-stranded RNA (dsRNA) pesticides trigger RNA interference by exogenous dsRNA specifically targeting pests and diseases. They can inhibit the expression of key genes in pathogens or pests, thereby achieving effective control of specific pests and diseases. DsRNA pesticides are environmentally friendly, with strong specificity and efficient gene silencing ability, while they have problems such as high production costs. Using engineering strains to produce dsRNA is a feasible strategy, whereas currently there is no cost-effective engineering strain for producing dsRNA. This article reviews the research progress and production strategies of using microorganisms to produce dsRNA, hoping to provide reference for dsRNA production.

Key words:RNA interference;dsRNA;engineering strain;chassis