精胺对霍山石斛类原球茎悬浮培养细胞生长和多糖合成的影响

Effect of Spermine on Cell Growth and Polysaccharide Production in Suspension Cultures of Protocorm-like Bodies from Dendrobium huoshanense

摘要:

研究了在添加外源精胺时，霍山石斛类原球茎细胞生长、多糖积累、主要营养物质消耗以及细胞内多胺含量的变化。结果表明，0.6mmol/L的精胺明显促进霍山石斛类原球茎细胞的生长和多糖的合成。细胞的比生长速率从0.046d^-1提高到0.054d^-1。培养30d时，类原球茎干重达32.4g DW/L，多糖总产量为2.46g/L ，分别是对照的1.32和1.31倍。添加外源精胺能够提高内源多胺的含量，同时，蔗糖酶和硝酸还原酶等相关代谢酶的活性增强，促进了碳、氮的吸收和利用。

Abstract:

The effect of outer spermine on cell growth, accumulation of polysaccharides and utilization of nutrient together with the intracellular polyamine contents were investigated in suspension cultures of protocorm-like bodies from Dendrobium huoshanense. The results indicated that spermine at 0.6mmol/L was the most effective in increasing cell growth and polysaccharide synthesis. The specific growth rate of cell increased from 0.046d^-1 to 0.054d^-1, and the maximum dry weight and polysaccharide production reached 32.4g DW/L and 2.46g/L respectively, which were 1.32-fold and 1.31-fold that of the control on day 30.The titres of intracellular free polyamines were higher in the cultures treated with spermine than that of the control. Invertase and nitrate reductase activities were found to increase significantly in the cultured cells treated with spermine, which was beneficial to the utilization of carbon and nitrogen source.