小鼠受精卵早期发育过程中PKB/Akt对p21蛋白表达及定位的影响

The Effect of Protein Kinase B on the Expression and Location of p21 in Early Development of Mouse Fertilized Eggs

摘要:

初步探讨在小鼠受精卵早期发育过程中PKB/Akt对p21 蛋白表达及定位的影响。通过显微操作技术注射野生型、持续激活型及激酶失活型的PKB的mRNA，用免疫荧光方法检测p21蛋白的细胞定位、Western blot 方法检测p21蛋白的表达。结果显示在注射不同形式的PKB mRNA 后p21蛋白的表达无明显差别，但是细胞定位发生改变，PKB被激活后，p21蛋白滞留在胞浆中。因而初步认为在小鼠受精卵中，PKB/Akt通过影响p21 的细胞定位而影响细胞周期的进程。

Abstract:

To investigate the effect of Protein kinase B on the expression and location of p21 in mouse early development. Immunopreciptation technology was used to detect the localization of p21 and Western blotting was used to analyze the expression of p21 after microinjecting mRNA of WT-PKB,myt-PKB and PKB-KD to mouse eggs. There was no obvious difference between the three kinds of mRNA in the expression of p21. But the cell localization altered. The p21 retain in cytoplasm after microjecting myt-PKB. In mouse fertilized egg PKB/Akt controls the cell cycle by changing the cell localization of p21.