添加CTAB促进吸水链霉菌产谷氨酰胺转胺酶

Improvement on the Activity of Microbial Transglutaminase with Streptomyces hygroscopicus by the Addition of Surfactant CTAB

摘要:

研究了添加十六烷基三甲基溴化铵（CTAB）对吸水链霉菌（Streptomyces hygroscopicus）合成谷氨酰胺转胺酶的影响。结果表明，添加CTAB可以提高发酵过程中谷氨酰胺转胺酶的酶活，摇瓶培养中，CTAB的最佳添加时间和添加量分别为32h和1%，发酵终了时，谷氨酰胺转胺酶酶活最高达5.04u/mL，比对照提高了21.8%。初步研究表明，CTAB的主要作用是促使谷氨酰胺转胺酶的酶原转化为成熟酶，因此，在发酵过程中添加适当浓度的CTAB，可使酶原快速、完全地转化为成熟的MTG，解除酶原的产物抑制作用，促进了细胞产酶。

Abstract:

Effect of CTAB addition on the accumulation of microbial transglutaminase(MTG) with Streptomyces hygroscopicus was investigated. The results showed that the addition of CTAB enhanced MTG accumulation, and the optimal addition time and concentration of CTAB were 32 h and 1%. The maximum MTG activity in the culture broth was 5.04u/mL and increased by 21.8% compared with the control. With the addition of CTAB, pro-MTG was activated to become MTG. CTAB could enhance the production of pro-MTG by relieving the product inhibition, and the accumulation of MTG was improved.