怀槐细胞悬浮培养的结构化动力学模型

Structure Kinetic Model of Maackia amurensis Cells in Suspension Culture

摘要:

为探明怀槐细胞生长、异黄酮染料木素合成与底物消耗间的关系，建立了怀槐细胞悬浮培养的结构化动力学模型。模型预测分析了胞内外的蔗糖代谢、胞内结构组分变化、胞内中间组分的变化、细胞呼吸损失以及胞内外异黄酮染料木素的合成情况。模型各参数灵敏度的分析表明kM_b1、k_b2 和k_p是最为灵敏的参数，其调节10%时，目标函数变化的最大比例分别达12.8%、4.61%和2.54%，其它参数对目标函数变化的影响均小于0.5%。该模型预测值与实验值具有较好的吻合性。

Abstract:

A structure kinetic model was established for describing the relationship among cell growth, genistein formation and substrate utilization in suspension culture of Maackia amurensis. In this model, sucrose uptake, structure components production, intermediate matter changes, cell respiration loss and secondary metabolite (genistein) production were all predicted. The parameter sensitivity test was indicated that intermediate matter self-catalysis rate constant (k_b1), structure component synthesis rate constant (k_b2) and secondary metabolite synthesis rate constant (kp)were the most sensitive parameters for this model. If k_b1, k_b2 or k_p are changed by 10%, the range of the aim function value would be changed by 12.8%, 4.61% and 2.54%, respectively. Adjustment of the other parameters only aroused a change of less than 0.5% in the aim function value. The predicted values were in good agreement with those obtained experimentally.