内芽孢杆菌属细菌（Paenibacillus daejeonensis）SS02抗菌蛋白的分离纯化和其性质的研究

Studies on Purification and Properties of Antagonistic Protein from Bacteria SS02 of Paenibacillus daejeonensis

摘要:

内芽孢杆菌属细菌（Paenibacillus daejeonensis）SS02，是一株能强烈抑制玉米纹枯菌（Rhizoctonia cerealis）、白色念珠菌 (Candidal vaginitis)等病害真菌的拮抗菌株。SS02菌株培养液经硫酸铵分级盐析，Sephadex G-75凝胶柱层析和DEAE-32纤维素柱层析分离纯化出一种抗菌蛋白，命名为SD22。蛋白电泳分析结果表明，此蛋白分子量为56000，等电点为6.4。此蛋白对热和紫外线稳定，对蛋白酶部分敏感。纯化后的SD22对玉米纹枯菌(Rhizoctonia cerealis)、油菜菌核病(Sclerotinia sclerotiorum)、苹果轮纹病菌（Physalospora piricala）、绿色木霉(Trichodema viride)、绿色粘帚霉（Gliocladium viride）、弯孢霉菌(Curvularia leaf spot)、镰刀霉菌(Fusarium sp.)、赤霉菌(Fusarium head blight)、球孢白僵菌（Beauveria bassiana）、大肠杆菌（Escherichia coli）、金黄色葡萄球菌（Staphylo-coccus aureus）、枯草芽孢杆菌（Bacillus subtilis）、白色念珠菌(Candidal vaginitis）、冬瓜枯萎菌（Fusarium oxysporum Schl.emend.Snyder & Hanse）等菌有很强的抑制作用。

Abstract:

The antifungal, anti-bacterical, anti-brine shrimp activities of SD22 isolated from Paenibacillus daejeonensis Bacteria SS02 were studied. The separation steps included ultracentrifugation, ultrafiltration and (NH₄)₂SO₄ fractional precipitation, further purification was performed by SephadexG-75 and DEAE-32 chromatography. Its molecular weight determined by SDS-PAGE was 56.0kD and its isoelectfic point was 6.4．SD22 was thermostable to some extent and stable to ultraviolet, but sensitive to some of the enzyme. SD22 could kill most pathogens from propagation, such asRhizoctonia cerealis,Sclerotinia sclerotiorum Physalospora piricala,Trichodema viride,Gliocladium viride,Curvularia leaf spot, Fusarium sp, Fusarium head blight, Beauveria Bassiana, Escherichia coli, Staphylo-coccus aureus, Bacillus subtilis,Candidal vaginitis, Fusarium oxysporum Schl.emend.Snyder&Hansem et al. The results will be helpful to find out a novel antifungal protein.