L-羟脯氨酸-Zn(Ⅱ)的配合机制及其抗氧化性研究

Coordination Mechanism and Antioxidant Activity of L-hydroxyproline Zinc(Ⅱ)

摘要:

以L-羟脯氨酸(Hyp)和ZnSO₄制备Hyp-Zn(Ⅱ)配合物，研究其配合机制及抗氧化能力。与L-羟脯氨酸相比，配合物在1100cm^－1处出现了一新的红外吸收峰，说明L-羟脯氨酸与Zn(Ⅱ)发生了配位作用；配合物的差热-热重图与L-羟脯氨酸相比，在290℃和375℃的吸热峰消失了，确证L-羟脯氨酸与Zn(Ⅱ)发生了配位作用；配合物核磁核磁共振图中3.5～3.9ppm的羧基氢和羟基氢的信号峰的消失，表明L-羟脯氨酸与Zn(Ⅱ)发生配合的位置是L-羟脯氨酸的α碳的羧基或γ碳的羟基氧；从配合物的原子力显微形貌相图可见数个L-羟脯氨酸围绕Zn(Ⅱ)形成的配合结构。透析实验结果证实L-羟脯氨酸与Zn(Ⅱ)配合的比例为4∶1（mol/mol）。上述测定和表征表明所形成配合物的分子式为Zn(Hyp)₄·H₂O。（Hyp）₄-Zn(Ⅱ)配合物抑制Zn(Ⅱ)产生的羟基自由基，抑制百分比为75.5%，其总抗氧化能力为80.167u/mL，抗超氧阴离子活力为53.19u/mL。

Abstract:

The coordination compound of L-hydroxyproline(Hyp)-Zn(Ⅱ) was synthesized with Hyp and zinc sulfate as raw materials in water medium, coordination Synthesizing Mechanism and Antioxidant Activity of Hyp-Zn(Ⅱ)coordination compound has been researched. Compared with Hyp, the infrared spectrogram of Hyp-Zn(Ⅱ) coordination compound emerge a new absorption peak at 1100cm^－1. Conclusion could be obtained that there exists a coordination effect between Hyp and ZnSO₄; TG and DSC curve of Hyp and Hyp-Zn(Ⅱ) coordination compound were analysed. Compared with Hyp, the peak of Hyp-Zn(Ⅱ) disappear at 290℃ and 375℃. This phenomenon confirmed the front conclusion; At the NMR graph of Hyp-Zn(Ⅱ)coordination compound, the disappearance of the α-carboxyl-hydrogen and α-hydroxyl- hydrogen's peak at 3.5～3.9ppm could indicate that combination's position of Hyp isα-carboxyl and α-hydroxyl; Structure of Hyp-Zn(Ⅱ) coordination compound were exosyndrome by the Atomic Force microscopy. It is showed that centr-atom Zn(Ⅱ) was surrounded by several Hyp at Hyp-Zn(Ⅱ) coordination compound's phase diagram; The proportion of Hyp-Zn(Ⅱ) coordination compound was determined by dialysis experiment, the proportion is 4∶1; Above-mentioned determination and exosyndrome indicated that the molecular formula of Hyp-Zn(Ⅱ) coordination compound is Zn(Hyp)₄·H₂O. The results indicated that the Hyp-Zn(Ⅱ) coordination compound can inhibit hydroxyl free radicals of Zn (Ⅱ), and the Percentage of Inhibition is 75.5% ;the total antioxidant activities of Hyp-Zn(Ⅱ) coordination compound is 80.167u/mL, the anti-superoxide activities of Hyp-Zn(Ⅱ) coordination compound is 53.19u/mL.