构建多顺反子表达载体的有效工具——FMDV 2A

An Efficient Tool for the Construction of Multiple-cistronic Vectors： FMDV 2A

摘要:

近年来肿瘤多基因治疗倍受关注，然而目前缺少一种有效的构建多顺子的工具。传统的构建多顺反子的工具，如IRES 等，由于存在结构大，上下游基因表达差异显著等缺陷，严重限制了其应用。FMDV 2A，因其具有结构小、剪切效率高等优点为多基因治疗带来了新的希望。主要介绍了FMDV 2A的特性、“剪切”活力及其在构建多顺反子载体中的应用。

Abstract:

Recently，cancer therapy with mutiple genes has been attached with great attention. However，at present there is no efficient tool to construct multiple-cistrons. The large sizes and the imbalance in expression of most traditional tools，such as ribosome entry sites (IRESes)，greatly block their wide employment in the construction of multiple cistronic gene therapy vectors. The self-cleaving peptide 2A from foot-and-mouth disease virus (FMDV) has a very small size，and more importantly，high cleavage activity in artifical bicistron，which bring new hope for mutiple genes therapy stategy. In this article，the characteristics and cleavage activities of FMDV 2A will be elucidated，and we further outline its applications in cancer gene therapy.