猪激素敏感脂酶和甘油三酯水解酶基因组织表达特性研究

Study on the Characteristics of Tissue Expression of Hormone Sensitive Lipase and Triacylglycerol Hydrolase in Pigs

摘要:

以八眉猪为研究对象，采用RT-PCR和Western blot方法对猪激素敏感酯酶（HSL）和甘油三酯水解酶（TGH）基因组织表达特点进行了研究。RT-PCR半定量检测显示，HSL基因的mRNA在检测的7种组织中都有表达，其中在脂肪组织表达量较高，中等程度表达于心脏、肝脏、肺、脾和肾脏。TGH基因在7种组织也均有表达，其中肝脏和脂肪组织表达量较高，心脏和肾脏次之，脾脏和肺脏表达量较低。Western blot检测显示，HSL基因在大网膜脂肪和皮下脂肪表达量最高，而在肾脏中没有检测到表达，其他组织中中度表达；TGH基因在大网膜脂肪、皮下脂肪、肝脏、肺脏和脾脏组织中表达，其中在脂肪组织和肝脏组织中表达量最高，而在心脏和肾脏中没有检测到表达。以上结果表明：HSL和TGH基因存在转录后调控，这可能与其在不同组织中的功能差异有关。

Abstract:

The specific expression of TGH and HSL genes in different tissues of Bamei pig was investigated by RT-PCR and Western blot in this study. The result of RT-PCR showed that the expression of HSL could be detected in all these seven tissues examined，and which was higher expressed in fat，lower in heart，liver，lung，spleen and kidney. Expression of TGH gene could also be detected in seven tissues，and higher in liver and fat，lower in heart and kidney and lowest in spleen and lung. The result of Western blot showed that，HSL gene was highest expressed in epiploica fat and subcutaneous fat，higher in other tissues，but couldn't be detected in kidney. Expression of TGH was detected in epiploica fat，subcutaneous fat，liver，lung and spleen，and highest in fat and liver，but it hadn't be found in heart and kidney. These results suggested that both HSL and TGH could be regulated by post-transcriptional，and their function was involved in different tissues.