一个水稻高效表达启动子Os252的分离

Isolation and Analysis of a High Expression Promoter in Rice

摘要:

植物基因的表达受启动子的控制，高效表达启动子的分离及功能分析不仅是植物基因工程研究的重要研究方面，也是表达调控研究的重要内容。根据EST数据克隆了一个预测在水稻茎中高效表达的启动子Os252。将该启动子与GUS基因构建成表达载体并转入水稻。转基因水稻PCR分析表明，GUS基因已经成功地整合进水稻基因组中。GUS组织化学分析表明，Os252能启动GUS基因在水稻叶、茎以及胚乳中表达。进一步GUS酶活性的测定表明，叶和胚乳中Os252启动子活性分别是35S启动子的1.9和2.5倍。由于Os252来自于水稻，在叶和胚乳中活性高于35S启动子，因此该启动子可望用于水稻基因工程研究。

Abstract:

The expression of plant gene is controlled by its promoter. The isolation and the function analysis of promoter are important for studying the genetic engineering and the regulation expression of plant genes. In this paper, we cloned a promoter, Os252, which was predicted to be highly expressed in the stem of rice from the EST database. After the construction of the Os252::GUS expression vector, it was transformed into rice. The integration of transgenes into transgenic rice genome was confirmed through PCR analysis. X-Gluc staining showed that Os252 can promote GUS gene expression in leaf, stem and matured seed. GUS enzyme activities driven by Os252 promoter in leaf and seed are about 190% and 250% of that driven by the 35S promoter. Thus, the Os252 promoter can be applied for rice genetic engineering.